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Greetings! Very useful advice in this particular article! The buffer issue is a challenge, but it needs to be resolved. The revision did provide a clearer picture. i.e high mol wt bands were clumped in top only. We have tried SB buffer and run it at 200 V but our 1KB DNA ladder did not resolve properly. The article is very professionally written. Just curious and interested in saving some time. Have you used the SB buffer in the DGGE apparatus? If so what have your run times and concentrations been? I have been using SB buffer with standard agarose gels but I am still using TAE buffer in our DGGE set up at 150V for 10h. Hi Stefan, I am new to this blog and stumbled upon it. Ultra-fast high-resolution agarose-electrophoresis of DNA and RNA using low-molarity conductive media. Analytical Biochemistry 2004 333:1-13.īrody JR, Kern SE. History and principles of conductive media for standard DNA electrophoresis. Sodium boric acid: A tris-less, cooler conductive medium for DNA electrophoresis. Buffer LB can be run at even higher voltages than SB, and is appropriate for separation of small DNA fragments.īrody JR, Kern SE. LA is also recommend for replacing MOPS in RNA separations. For longer DNA fragments (>3 kb), buffer LA should be used. We are running gels with this buffer at 300V. The authors recommend that for standard electrophoresis applications, buffer SB be used. The advantages of these buffers are multiple (a) they generate much less heat than TAE or TBE buffers, (b) they can be run at much high voltages (>300V in some cases) without fear of gels melting, (c) they can be much cheaper than TAE or TBE, (d) they do not interfere with standard applications such as band excision, and (e) they last longer before needing to be replaced.
(3) LB Buffer – 1 mM lithium boric acid (lithium hydroxide pH buffered to pH ~8.5 with boric acid) 20X concentrate should have a pH of 8.0 → for more details see the manuscript below) (1) SB Buffer – 10 mM sodium boric acid (1X working solution is either 5 mM disodium borate decahydrate or 10 mM NaOH adjusted to pH 8.5 with boric acid up to 50X concentrates can be made. Also found below is a link to the company “Faster-better-media” selling these buffers. Links to the relevant manuscripts are below.
I would like to advocate the use of three new electrophoresis buffers.
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I thought about sticking a smart doorbell outside (like the Ring), but I don't think I can do that either since I share the small porch with 3 other units.ĭoes anyone have suggestions on how to go about being able to see who's at my door? I expect to have to open some doors to sign for packages, and I would really like to see who's there before deciding to open or not.I am revising this submission to be a little clearer. Based on this long response from him, I doubt he'll allow me to drill anything for a new peephole or door camera.
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I don't think the landlord is legally required to install a peephole because of the windows, and he advised me not to install a chainlock "in case an emergency happens where firemen have to break open the door because they can't get in" and leave me with a thousand dollar door bill.
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Instead it has two tiny windows at the top of the door that are too high and small to show me anything but the sky. I'd really appreciate your help as I'm living on my own for the first time!Īpt is a second floor unit with a single external door (stairs inside), and this door doesn't have a peephole. I saw a couple of other threads on peephole alternatives but couldn't find one helpful for my new rental.
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